Free Flow Electrophoresis of Chloroplasts
نویسندگان
چکیده
منابع مشابه
Free flow electrophoresis of chloroplasts.
Highly purified intact chloroplasts were isolated from spinach (Spinacia oleracea L.) leaves by free flow electrophoresis. Morphological and biochemical studies showed that the fraction enriched in intact chloroplasts has a higher protein to chlorophyll ratio and a higher linolenic acid content than the broken organelles of the other fraction. The intact chloroplasts prepared by electrophoresis...
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A separation of spinach chloroplasts in vitro into fractions according to size (volume) and activity (light-dependent shrinkage and NADP reduction) has been achieved by stable-flow free boundary sedimentation-electrophoresis. The salient features of this chloroplast study are: (a) separation is achieved within 30 min; (b) only small density gradients are required, thus minimizing osmotic effect...
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Small-volume continuous-flow synthesis (small-volume CFS) offers a number of benefits for use in small-scale chemical production and exploratory chemistry. Typically, small-volume CFS is followed by discontinuous purification; however, a fully continuous synthesis-purification combination is more attractive. Milli free-flow electrophoresis (mFFE) is a promising continuous-flow purification tech...
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We propose an electrophoretic technique combining the use of a series of dielectric traps controlled by an ac electric field and conventional continuous field free-flow electrophoresis. From the theoretical model that we describe, one can expect, for DNA electrophoresis, an improvement of one to two orders of magnitude in the selectivities or experimental durations.
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For sorting, cells or cellular components can specifically be labeled by antibody-coated magnetic beads. We have developed a device for continuous magnetic sorting based on the flow-chamber of a free-flow electrophoresis system. Magnetically labeled particles are injected into a given continuously flowing chamber buffer and pass an inhomogeneous magnetic field, configurated perpendicular to the...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1978
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.61.3.465